F4/80 Allophycocyanin Antibody | Rockland Immunochemicals 200-526-L55 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

F4/80 Allophycocyanin Antibody

catalog :

200-526-L55

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

BM8.1

reactivity :

mouse

application :

flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-526-L55

Name :

Anti-F4/80 (RAT) Allophycocyanin Conjugated Monoclonal Antibody - 200-526-L55

Display Name :

F4/80 Allophycocyanin Antibody

Application Note :

Anti-F4/80 is useful for Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

BM8.1

Concentration Value :

0.2 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

APC

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

10 µL/10^6 cells (0.1 µg)

Fluorochrome Protein Value :

1-2

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG2a

Gene Name :

Adgre1

Host Animal :

Rat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-F4/80 Antibody (Monoclonal) was produced by repeated immunizations with F4/80 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Allophycocyanin conjugated F4/80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse F4/80. Cross reactivity with F4/80 from other sources has not been tested. Anti-F4/80 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Species Reactivity :

Mouse

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Subclass :

IgG2a kappa

Synonyms :

EMR1, Ly71, EGF-TM7, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, Gpf480, Adgre1

Target Species :

Mouse

Background :

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,

Category :

Primary Antibodies

Conjugation Name :

Allophycocyanine

UniProt :

Q61549

NCBI :

NP_034260.1

Primary Image Name :

Flow Cytometry of anti-F4/80 APC - 200-526-L55

more info or order :

Rockland Immunochemicals product webpage