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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Ly-6G (Gr-1) Allophycocyanin Antibody
catalog :
200-526-L54
quantity :
100 µg
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
RB6-8C5
reactivity :
mouse
application :
immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-526-L54
Name :
Anti-Ly-6G (Gr-1) (RAT) Allophycocyanin Conjugated Monoclonal Antibody - 200-526-L54
Display Name :
Ly-6G (Gr-1) Allophycocyanin Antibody
Application Note :
Anti-Ly-6G (Gr-1) is useful for Immunohistochemistry, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
RB6-8C5
Concentration Value :
0.2 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
APC
Size :
100 µg
Default Unit :
µg
Flow Cytometry Dilution :
10 µL/10^6 cells (0.1 µg)
Fluorochrome Protein Value :
1-2
Immunohistochemistry Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG2b
Gene Name :
Gr-1
Host Animal :
Rat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Ly-6G (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with Ly-6G/Ly-6C (Gr-1) antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Allophycocyanin conjugated Ly-6G (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G (Gr-1). Cross reactivity with Ly-6G (Gr-1) from other sources has not been tested. Anti-Ly-6G (Gr-1) is conjugated with APC under optimal conditions and the solution is free of unconjugated APC. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C.
Species Reactivity :
Mouse
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG2b kappa
Synonyms :
Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C
Target Species :
Mouse
Background :
The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,Immunoprecipitation,
Category :
Primary Antibodies
Conjugation Name :
Allophycocyanine
NCBI :
NP_001238984.1
Primary Image Name :
Flow Cytometry of anti-Ly6G (Gr1) APC - 200-526-L54
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.