CD86 Phycoerythrin Antibody | Rockland Immunochemicals 200-508-N90 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD86 Phycoerythrin Antibody

catalog :

200-508-N90

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

GL-1

reactivity :

mouse

application :

immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-508-N90

Name :

Anti-CD86 (RAT) Phycoerythrin Conjugated Monoclonal Antibody - 200-508-N90

Display Name :

CD86 Phycoerythrin Antibody

Application Note :

Anti-CD86 is useful for Immunohistochemistry, Immunofluorescence, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

GL-1

Concentration Value :

0.2 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

APC

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

10 µL/10^6 cells (0.1 µg)

Fluorochrome Protein Value :

1-2

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG2a

Gene Name :

Cd86

Host Animal :

Rat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Phycoerythrin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD86. Cross reactivity with CD86 from other sources has not been tested. Anti-CD86 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Species Reactivity :

Mouse

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Subclass :

IgG2a kappa

Synonyms :

T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86, Cd86

Target Species :

Mouse

Background :

Anti-CD86 reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,IF Microscopy,

Category :

Primary Antibodies

Conjugation Name :

Allophycocyanine

UniProt :

P42082

NCBI :

NP_062261.3

Primary Image Name :

Flow Cytometry of anti-CD86 PE - 200-508-N90

more info or order :

Rockland Immunochemicals product webpage