product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD45.1 Phycoerythrin Antibody
catalog :
200-508-N85
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
RPE
clone name :
A20
reactivity :
mouse
application :
immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-508-N85
Name :
Anti-CD45.1 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-508-N85
Display Name :
CD45.1 Phycoerythrin Antibody
Application Note :
Anti-CD45.1 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
A20
Concentration Value :
0.2 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
RPE
Size :
100 µg
Default Unit :
µg
Flow Cytometry Dilution :
10 µL/10^6 cells (0.1 µg)
Fluorochrome Protein Value :
1-2
Immunohistochemistry Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG2a
Gene Name :
Ptprc
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Phycoerythrin conjugated CD45.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.1. Cross reactivity is observed with human and Feline. Cross-reactivity with CD45.1 from other sources has not been tested. Anti-CD45.1 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Species Reactivity :
Mouse
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG2a kappa
Synonyms :
Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Target Species :
Mouse
Background :
The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,Immunoprecipitation,
Category :
Primary Antibodies
Conjugation Name :
R-Phycoerythrin (RPE)
UniProt :
P06800
Primary Image Name :
Flow Cytometry of anti-CD45.1 PE - 200-508-N85
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.