product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD25 Phycoerythrin Antibody
catalog :
200-508-M73
quantity :
100 µg
clonality :
monoclonal
host :
rat
conjugate :
RPE
clone name :
PC61
reactivity :
mouse
application :
flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-508-M73
Name :
Anti-CD25 (RAT) Phycoerythrin Conjugated Monoclonal Antibody - 200-508-M73
Display Name :
CD25 Phycoerythrin Antibody
Application Note :
Anti-CD25 is useful for IF and Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
PC61
Concentration Value :
0.2 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
RPE
Size :
100 µg
Default Unit :
µg
Flow Cytometry Dilution :
0.2-1 µg/1x10^6 cells
Fluorochrome Protein Value :
1-2
IF Microscopy Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG1
Gene Name :
Il2r
Host Animal :
Rat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent cytolytic mouse T-cell clone B6.1.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Phycoerythrin conjugated CD25 Monoclonal Antibody was Protein G Purified and is directed against mouse CD25. Reactivity is observed against mouse CD25. Cross reactivity with CD25 from other sources has not been tested.
Species Reactivity :
Mouse
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG1 lambda
Synonyms :
Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25 Antibody
Target Species :
Mouse
Background :
The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naive or conventional T cells which are CD25.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,IF Microscopy,
Category :
Primary Antibodies
Conjugation Name :
R-Phycoerythrin (RPE)
UniProt :
P01590
NCBI :
NP_032393.3
Primary Image Name :
Flow Cytometry - Rat anti-MOUSE CD25 PE
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.