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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Ly-6G/Ly-6C (Gr-1) Phycoerythrin Antibody
catalog :
200-508-L54
quantity :
200 µg
clonality :
monoclonal
host :
rat
conjugate :
RPE
clone name :
RB6-8C5
reactivity :
mouse
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-508-L54
Name :
Anti-Ly-6G/Ly-6C (Gr-1) (RAT) Phycoerythrin Conjugated Monoclonal Antibody - 200-508-L54
Display Name :
Ly-6G/Ly-6C (Gr-1) Phycoerythrin Antibody
Application Note :
Anti-Ly-6G/Ly-6C (Gr-1) is useful for Immunohistochemistry, Immunoprecipitation, Western Blot, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
RB6-8C5
Concentration Value :
0.2mg/mL
Concentration Definition :
Sufficient to run approximately 100 tests
Conjugation :
RPE
Size :
200 µg
Default Unit :
µg
Flow Cytometry Dilution :
10 µL/10^6 cells (0.1 µg)
Fluorochrome Protein Value :
1-2
Immunohistochemistry Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG2b
Gene Name :
Gr-1
Host Animal :
Rat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Ly-6G/Ly-6C (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with raised against granulocytes of mouse origin.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Phycoerythrin conjugated Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G/Ly-6C (Gr-1). Cross reactivity with Ly-6G/Ly-6C (Gr-1) from other sources has not been tested. Anti-Ly-6G/Ly-6C (Gr-1) is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Species Reactivity :
Mouse
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG2b kappa
Synonyms :
Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C
Target Species :
Mouse
Background :
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
R-Phycoerythrin (RPE)
NCBI :
NP_001238984.1
Primary Image Name :
Flow Cytometry of anti-Ly-6G/Ly-6C (Gr-1) PE - 200-508-L54
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.