CD62L Fluorescein Antibody | Rockland Immunochemicals 200-502-N87 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD62L Fluorescein Antibody

catalog :

200-502-N87

quantity :

500 µg

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

MEL-14

reactivity :

mouse

application :

immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-502-N87

Name :

Anti-CD62L (RAT) Fluorescein Conjugated Monoclonal Antibody - 200-502-N87

Display Name :

CD62L Fluorescein Antibody

Application Note :

Anti-CD62L is useful for Immunohistochemistry, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

MEL-14

Concentration Value :

0.5mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

FITC

Size :

500 µg

Default Unit :

µg

Flow Cytometry Dilution :

10 µL/10^6 cells (0.1 µg)

Fluorochrome Protein Value :

2-8

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG2a

Gene Name :

Sell

Host Animal :

Rat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Fluorescein conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L. Cross reactivity with CD62L from other sources has not been tested. Anti-CD62L is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Species Reactivity :

Mouse

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Subclass :

IgG2a kappa

Synonyms :

L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22

Target Species :

Mouse

Background :

Anti-CD62L antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte rolling on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,Immunoprecipitation,

Other :

Sufficient to run approximately 500 tests

Category :

Primary Antibodies

Conjugation Name :

Fluorescein (FITC)

UniProt :

P18337

NCBI :

NP_035476.1

Primary Image Name :

Flow Cytometry of anti-CD62L FITC - 200-502-N87

more info or order :

Rockland Immunochemicals product webpage