CD45R (B220) Fluorescein Antibody | Rockland Immunochemicals 200-502-N84 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD45R (B220) Fluorescein Antibody

catalog :

200-502-N84

quantity :

500 µg

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

RA3-6B2

reactivity :

mouse

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-502-N84

Name :

Anti-CD45R (B220) (RAT) Phycoerythrin Conjugated Monoclonal Antibody - 200-502-N84

Display Name :

CD45R (B220) Fluorescein Antibody

Application Note :

Anti-CD45R (B220) is useful for Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, and Western Blot using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

RA3-6B2

Concentration Value :

0.5mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

FITC

Size :

500 µg

Default Unit :

µg

Flow Cytometry Dilution :

10 µL/10^6 cells (0.1 µg)

Fluorochrome Protein Value :

2-8

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG2a

Gene Name :

Ptprc

Host Animal :

Rat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD45R (B220) Antibody (Monoclonal) was produced by repeated immunizations with CD45R antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Fluorescein conjugated CD45R (B220) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45R. Cross reactivity is observed with human and Feline. Cross reactivity with CD45R from other sources has not been tested. Anti-CD45R is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Species Reactivity :

Mouse/Human

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Subclass :

IgG2a kappa

Synonyms :

Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

Target Species :

Mouse

Background :

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Other :

Sufficient to run approximately 500 tests

Category :

Primary Antibodies

Conjugation Name :

Fluorescein (FITC)

UniProt :

P06800

Primary Image Name :

Flow Cytometry of anti-CD45R FITC - 200-502-N84

more info or order :

Rockland Immunochemicals product webpage