Lipoamide Dehydrogenase Antibody | Rockland Immunochemicals 200-4160-0100 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Lipoamide Dehydrogenase Antibody

catalog :

200-4160-0100

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

pigs

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-4160-0100

Name :

Anti-LIPOAMIDE DEHYDROGENASE (Porcine Heart) (RABBIT) Antibody - 200-4160-0100

Display Name :

Lipoamide Dehydrogenase Antibody

Application Note :

Anti-LIPOAMIDE DEHYDROGENASE has been assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:20,000

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:500 - 1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

DLD LAD

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Lipoamide Dehydrogenase [Porcine Heart]

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-LIPOAMIDE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]. Cross reactivity against Lipoamide Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

Porcine

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase

Target Species :

Swine

Background :

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of the alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). In monomeric form it has additional moonlighting function as serine protease. It is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P09623

NCBI :

118675

more info or order :

Rockland Immunochemicals product webpage