antibody

Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated

200-406-385

100 µg

polyclonal

domestic rabbit

biotin

western blot, ELISA

Anti-MALTOSE BINDING PROTEIN (MBP) EPITOPE TAG (RABBIT) Antibody Biotin Conjugated - 200-406-385

Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated

Anti-MBP Biotin Conjugated Antibody is optimally suited for monitoring the expression of MBP tagged fusion proteins.  As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope.  The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions.  Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli.  By Western blot, a band is seen at ~ 42 kDa representing MBP.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

1.0 mg/mL

by UV absorbance at 280 nm

BAC

100 µg

µg

1:10,000-1:50,000

1:1,000-1:5,000

Expiration date is one (1) year from date of opening.

IgG

Rabbit

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein.

Ambient

Lyophilized

0.01% (w/v) Sodium Azide

This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag.  To date this antibody has reacted with all MBP tagged proteins so far tested.  In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.

Restore with deionized water (or equivalent)

100 µL

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

rabbit anti-MBP Epitope Tag Antibody biotin conjugated, rabbit anti-Maltose Binding Protein Antibody biotin conjugation

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

MBP

Recombinant Protein

No

No

ELISA,Western Blot,

User Optimized

GFP and Epitopes

Biotin

Rb Anti-Maltose Binding Protein Antibody - Western Blot