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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Beta-2-Microglobulin Antibody Peroxidase Conjugated
catalog :
200-403-141
quantity :
500
price :
https://www.rockland.com/search/?searchString=200-403-141
clonality :
polyclonal
host :
domestic rabbit
conjugate :
HRP
reactivity :
human
application :
western blot, ELISA, immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
image
image 1 :
Rockland Immunochemicals 200-403-141 image 1
Immunohistochemistry of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
product information
Catalog Number :
200-403-141
Name :
Anti-BETA-2-MICROGLOBULIN (Human Urine) (RABBIT) Antibody Peroxidase Conjugated - 200-403-141
Display Name :
Beta-2-Microglobulin Antibody Peroxidase Conjugated
Application Note :
Anti-beta-2-Microglobulin Peroxidase antibody has been tested in ELISA and western blotting, and is suitable for IF and IHC. Researchers should determine optimal titers for applications that are not stated below.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
5.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
HRP
Size :
500
Default Unit :
µg
ELISA Dilution :
1:2,000 - 1:10,000
Immunohistochemistry Dilution :
1:200 - 1:1,000
IF Microscopy Dilution :
1:200
Western Blot Dilution :
1:500 - 1:2,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
B2M
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Purity and Specificity :
Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine).  Cross reactivity against b2-Microglobulin from other sources is unknown.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Species Reactivity :
Human
Tested Applications :
ELISA; WB
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-Beta-2-Microglobulin Antibody Peroxidase Conjugation, HRP conjugated Beta-2-Microglobulin Antibody, B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody
Target Species :
Human
Background :
Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Peroxidase (Horseradish)
2024 Price :
$525.00
UniProt :
P61769
NCBI :
NP_004039.1
Primary Image Name :
Anti-Beta-2-Microglobulin Antibody - Immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.