5-caC Antibody | Rockland Immunochemicals 200-401-X50 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

5-caC Antibody

catalog :

200-401-X50

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-X50

Name :

Anti-5-Carboxylcytosine (RABBIT) Antibody - 200-401-X50

Display Name :

5-caC Antibody

Application Note :

Anti-5caC Antibody is suitable for Dot Blots, Immunofluorescence and Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-5caC Antibody was produced in rabbits by repeated immunizations with 5-carboxylcytosine (5caC).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-5caC Antibody was purified by Protein A purification. Cross reactivity with other species was not tested but a wide range is presumed.

Species Reactivity :

Human, Mouse, Other (wide range)

Stabilizer :

30% Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

5ca-CMP monophosphate

Background :

Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases. Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base. Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5caC Antibody is ideal for research in Genetics and Epigenetics.

Immunogen Type :

Other

Low Endotoxin :

Sample Size :

Other :

Dot Blot: 1:500 - 1:1,000

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

Primary Image Name :

Dot Blot of anti-5-Carboxylcytosine antibody

more info or order :

Rockland Immunochemicals product webpage