p53 K372 me1 Antibody | Rockland Immunochemicals 200-401-X38 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p53 K372 me1 Antibody

catalog :

200-401-X38

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-X38

Name :

Anti-p53 K372 me1 (RABBIT) Antibody - 200-401-X38

Display Name :

p53 K372 me1 Antibody

Application Note :

Anti-p53 K372 me1 Antibody is suitable for Chromatin Immunoprecipitation and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47kDa in the appropriate cell lysate or extract.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

2.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ChIP Dilution :

5 μg per IP

Western Blot Dilution :

1:200

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

TP53

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-p53 K372 me1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human p53 methylated at K372.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide and 0.05% ProClin 300

Purity and Specificity :

Anti-p53 K372 me1 Antibody was purified by Protein G chromatography. Cross reactivity with other species was not tested.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, transcription factor p53, TRP53, LSF1

Target Species :

Human

Background :

The transcription factor p53 is a tumor suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. p53 is a short-lived protein which is activated and stabilized via different post-translational modifications. One of these modifications, methylation of lysine 372 has been shown to positively affect the stability of p53. Mutations in p53 are involved in a vast majority of human cancers. Anti-p53 K372 me1 Antibody is ideal for research in Gene Expression, Cell Cycle Control, Apoptosis and Cancer.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Methylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Chip,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P04637

NCBI :

NP_000537.3

Primary Image Name :

Western Blot of anti-p53 K372 me1 antibody

more info or order :

Rockland Immunochemicals product webpage