EzH2 Antibody | Rockland Immunochemicals 200-401-W53 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

EzH2 Antibody

catalog :

200-401-W53

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-W53

Name :

Anti-EZH2 (RABBIT) Antibody - 200-401-W53

Display Name :

EzH2 Antibody

Application Note :

Anti-EZH2 Antibody is suitable for Chromatin Immunoprecipitation, Immunochemistry and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85 kDa in the appropriate cell lysate or extract.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ChIP Dilution :

2.5 μg per IP

Immunohistochemistry Dilution :

1:100 - 1:500

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

EZH2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-EZH2 Antibody was produced in rabbits by repeated immunizations containing an amino acid sequence from the N-terminus of mouse EZH2.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide and 0.05% ProClin 300

Purity and Specificity :

Anti-EZH2 Antibody was purified by Protein G chromatography. Cross reactivity with other species was not tested.

Species Reactivity :

Mouse, Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2

Target Species :

Human

Background :

EZH2 is a histone-lysine methyltransferase which methylates ‘Lys-9’ and ‘Lys-27’ of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and internal nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer. Anti-EZH2 Antibody is ideal for research in Epigenetics, Gene Expression, Cell Biology and Cancer.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

Chip,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q15910

NCBI :

NP_001190176.1

Primary Image Name :

Chromatin Immunoprecipitation of Anti-EZH2

more info or order :

Rockland Immunochemicals product webpage