HP1 alpha, beta, gamma Antibody | Rockland Immunochemicals 200-401-V35 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

HP1 alpha, beta, gamma Antibody

catalog :

200-401-V35

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-V35

Name :

Anti-HP1 alpha, beta, gamma (RABBBIT) Antibody - 200-401-V35

Display Name :

HP1 alpha, beta, gamma Antibody

Application Note :

Anti-HP1 α, β, g Antibody is suitable for Chromatin Immunoprecipitation, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20 kDa (bands for the alpha, beta, and gamma isoforms are slightly different) in the appropriate cell lysate or extract.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

2.0 µg/µL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ChIP Dilution :

4 μg/ChIP

IF Microscopy Dilution :

1:500

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CBX1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-HP1 α, β, g Antibody was produced in rabbits by repeated immunization with a full length recombinant protein of human HP1 beta. The antibody also recognizes the alpha and gamma isoforms.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide and 0.05% ProClin 300

Purity and Specificity :

Anti-HP1 alpha, beta, gamma Antibody was purified by Protein G chromatography. Cross-reactivity with HP1 α, β, G from species other than human has not been determined.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

Chromobox protein homolog 1, HP1Hsbeta, Heterochromatin protein 1 homolog beta, HP1-beta, Heterochromatin protein p25, M31, Modifier 1 protein, p25 beta

Target Species :

Human

Background :

HP1 alpha, beta and gamma (UniProt/Swiss-Prot entry P45973, P83916 and Q13185) are components of heterochromatin. They recognize and bind histone H3 tails methylated at ‘Lys-9’, leading to epigenetic repression of transcription. HP1 alpha, beta and gamma also interact with lamin B receptor (LBR), thereby contributing to the association of heterochromatin with the innernuclear membrane. Anti-HP1 α, β, g Antibody is ideal for research in Chromatin Remodeling and Epigenetics.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

Chip,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P83916

NCBI :

NP_001120700

Primary Image Name :

Immunofluorescence Microscopy of anti-HP1 alpha, beta, gamma antibody

more info or order :

Rockland Immunochemicals product webpage