product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
AGAP4 Antibody
catalog :
200-401-P07
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
Catalog Number :
200-401-P07
Name :
Anti-AGAP4 (RABBIT) Antibody - 200-401-P07
Display Name :
AGAP4 Antibody
Application Note :
Anti-AGAP4 antibody has been tested for use in western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73kDa in size corresponding to AGAP4 by western blotting in the appropriate cell lysate or extract.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.04mg/ml
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:10,000-1:20,000
Western Blot Dilution :
1:1000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
AGAP4
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-AGAP4 antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant His-tagged AGAP4 protein.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-AGAP4 antibody was purified from monospecific antiserum by protein A affinity purification and the His-tag was removed by cross adsorption. An immunoblot assay detects overexpressed recombinant AGAP4.
Species Reactivity :
Human
Storage :
Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-AGAP4 antibody, AGAP-4, AGAP 4, CTGLF1, MRIP2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 4, Centaurin-gamma-like family member 1
Target Species :
Human
Background :
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). The AGAPs are the largest subgroup of the ArfGAP family with 11 genes in humans. AGAP4 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 4) is a GTPase activating protein. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. AGAP4 and other AGAP proteins likely function in the secretory pathway, which could explain a link with the invasive behavior of cancer cells. Reliable antibodies would be invaluable for studies of the physiologic function of the proteins and how is may be linked to cancer cell invasive behavior. Anti-AGAP4 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q96P64
NCBI :
NP_597703.2
Primary Image Name :
Western Blot - anti-AGAP4
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.