SARS-CoV-2 Nucleocapsid (N) Protein Antibody | Rockland Immunochemicals 200-401-MS4 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

SARS-CoV-2 Nucleocapsid (N) Protein Antibody

catalog :

200-401-MS4

quantity :

500 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

SARS-CoV-2

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Western Blot Results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB-210-0500). Lane 2: SARS CoV-2 N protein (10ng). Primary Antibody: Anti-SARS CoV-2 N Protein at 1µg/mL overnight for 2 days at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugated Antibody (p/n 611-1302) at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073) for 1hr at RT. Predicted MW: ~47kDa; ~55kDa for glycosylated CoV-2-His-tag. Observed MW: ~63kDa. Other bands are due to overload of protein. Exposure: 1 sec.

product information

Catalog Number :

200-401-MS4

Name :

Anti-SARS-CoV-2 Nucleocapsid (N) Protein (RABBIT) Antibody - 200-401-MS4

Display Name :

SARS-CoV-2 Nucleocapsid (N) Protein Antibody

Application Note :

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

5.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

500 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Western Blot Dilution :

1:1000-1:2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This protein A purified antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. Cross reactivity with homologues from other sources has not been determined.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

500 µL

Species Reactivity :

SARS-CoV-2

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2

Target Species :

SARS

Background :

SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2 or COVID-19) is related to SARS-CoV, MERS, and four milder coronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive-strand RNA virus that consists of four structural proteins: spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein. The spike protein is the most important surface protein of coronavirus. SARS-CoV-2 has a high affinity binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial. ACE2 is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems. The nucleocapsid protein is a most abundant protein of coronavirus. The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Anti-SARS-CoV-S Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

NCBI :

MN908947.3

Primary Image Name :

Western Blot Results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage