ATG8 Antibody | Rockland Immunochemicals 200-401-H57 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ATG8 Antibody

catalog :

200-401-H57

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-H57

Name :

Anti-ATG8 (RABBIT) Antibody - 200-401-H57

Display Name :

ATG8 Antibody

Application Note :

Anti-ATG8 antibody is suitable for use in IHC (p) and WB. Expect a band approximately 16 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

5-10 µg/mL

Western Blot Dilution :

0.25-1 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

MAP1LC3A

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

ATG8 Antibody was produced from whole rabbit serum prepared by repeated immunizations with at the n-terminus portion of human ATG8.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-ATG8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-ATG8 from Opossum and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-ATG8 from other sources has not been determined.

Species Reactivity :

Human, Mouse, Rat

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

Activating transcription factor 8

Target Species :

Human

Background :

Anti-ATG8 antibody detects human ATG8. MAP1A and MAP1B are microtubule-associated proteins made up of a heavy chain and multiple light chain components. One such light chain component associated with these proteins is ATG8. ATG8 (also called MAP1LC3A and APG8), an ~16KDa mammalian homolog of yeast Atg8, is a cytoplasmic protein that plays an essential role in the process and regulation of autophagy. ATG8 is an evolutionarily conserved protein that belongs to LC3 family. ATG8 interacts with various proteins in the cytoplasm including mammalian homologues of Atg4, Atg7, Atg3 and Atg12 and gets modified into LC3-I and LC3-II respectively. Both of these forms are suggested to be involved in the formation and intracellular trafficking of autophagosomes in mammalian cells. Anti-ATG8 antibody are ideal for investigators invovled in apoptosis research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9H492

NCBI :

NP_115903.1

Primary Image Name :

ATG8 Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage