CRASP-1 Antibody | Rockland Immunochemicals 200-401-C18 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CRASP-1 Antibody

catalog :

200-401-C18

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Borreliella burgdorferi B31

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-C18

Name :

Anti-CRASP-1 (RABBIT) Antibody - 200-401-C18

Display Name :

CRASP-1 Antibody

Application Note :

This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 26.9 kDa in size corresponding to Borrelia burgdorferi CRASP-1 protein by Western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

>1:5,000

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CRASP1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-1 protein.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi CRASP-1 protein. A BLAST analysis was used to suggest reactivity with CRASP-1 from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. spielmanii, afzelii, and valaisiana sources based on 98% homology. Cross-reactivity with CRASP-1 from other sources has not been determined.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

Borrelia burgdorferi

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-CRASP-1 Antibody, Borrelia burgdorferi CRASP-1, CRASP1, CRASP 1, Complement regulator acquiring protein 1

Target Species :

Borrelia burgdorferi

Background :

CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q66ZC1

NCBI :

NP_045741

Primary Image Name :

Anti-CRASP-1 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage