TEV Protease Antibody | Rockland Immunochemicals 200-401-B91 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

TEV Protease Antibody

catalog :

200-401-B91

quantity :

100

price :

499.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Tobacco etch virus

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-B91

Name :

Anti-TEV Protease (RABBIT) Antibody - 200-401-B91

Display Name :

TEV Protease Antibody

Application Note :

This protein A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful to detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

1:1,500-1:27,000

Western Blot Dilution :

1:500

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. Assay by western blot showed no reactivity to MBP.

Species Reactivity :

TEV

Tested Applications :

ELISA; WB

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-TEV Protease Antibody, Tobacco Etch Virus, tobacco etch potyvirus, TEV, TEV Protease

Target Species :

Tobacco Etch Virus

Background :

TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S). Cleavage occurs between Q and G or Q and S. The structure of TEV protease is similar to that of the serine protease family. Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds. The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used. The strain used is the auto inactivation-resistant mutant S219V. The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2025 Price :

499.00 USD

UniProt :

P04517

NCBI :

NP_734212.1

Primary Image Name :

Anti-TEV protease, western blot

more info or order :

Rockland Immunochemicals product webpage