IL13 Antibody | Rockland Immunochemicals 200-401-B55 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IL13 Antibody

catalog :

200-401-B55

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

pigs

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-B55

Name :

Anti-Swine IL-13 (RABBIT) Antibody - 200-401-B55

Display Name :

IL13 Antibody

Application Note :

IL-13 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13.2 kDa in size corresponding to swine IL-13 protein by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:11,000

Western Blot Dilution :

1.5 to 2 µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

IL13

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for swine IL-13 protein. A BLAST analysis was used to suggest reactivity with IL-13 from swine sources based on 100% homology with the immunizing sequence. The following homologies may indicate chances of cross-reactivity when amino acid similarity and not just exact sequence is accounted: 89-90% homology to IL-13 from sheep, dolphin, beluga, and bovine, 83-88% to human and certain monkey IL-13, 80-82% to dusky titi, macaque, baboon, mangabey, dog, llama, camel, and galago, 75-78% to horse, and 67-72% to rat, mouse, and gerbil. Cross-reactivity with IL-13 from other sources has not been determined.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

swine, sheep, bovine

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Interleukin-13 antibody, rabbit anti-IL-13 antibody, IL13, Interleukin13, IL 13, Interleukin 13

Target Species :

Swine

Background :

Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one IL-13-specific binding chain. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6. In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells. In mice, deletion of IL-13 does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. Deletion of IL-4 abrogates these responses. IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. Anti-IL-13 antibody antibody is ideal for investigators involved in Cancer and Immunology research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

B3GDZ5

NCBI :

189176151

Primary Image Name :

Anti-Swine IL-13 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage