IRAK Antibody | Rockland Immunochemicals 200-401-951 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IRAK Antibody

catalog :

200-401-951

quantity :

100 µg

price :

369.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-951

Name :

Anti-IRAK (RABBIT) Antibody - 200-401-951

Display Name :

IRAK Antibody

Application Note :

This IgG fraction antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to IRAK protein by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:20,000

Immunohistochemistry Dilution :

1:100 - 1:500

Immunoprecipitation Dilution :

2-4 ug per sample

Western Blot Dilution :

1:500 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

IRAK1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human IRAK protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This IgG fraction antibody is directed against human IRAK protein. The product was purified from monospecific antiserum by DEAE chromatography. A BLAST analysis was used to suggest cross reactivity with IRAK protein from human, mouse and rat based on 100% homology with the immunizing sequence. No cross reactivity occurs against IRAK-2. Reactivity against homologues from other sources is not known.

Species Reactivity :

human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Il1rak antibody, Interleukin 1 receptor associated kinase 1 antibody, Interleukin 1 receptor associated kinase 2 antibody, IRAK1 antibody, IRAK2 antibody, mPLK antibody, Pelle antibody, Plpk antibody

Target Species :

Human

Background :

IRAK (Interleukin-1 receptor-associated kinase 1). Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and LPS. A serine/ threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-kB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

P51617

NCBI :

68800243

Primary Image Name :

Anti-IRAK antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage