ADAM10 Antibody | Rockland Immunochemicals 200-401-949 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ADAM10 Antibody

catalog :

200-401-949

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-949

Name :

Anti-ADAM10 (RABBIT) Antibody - 200-401-949

Display Name :

ADAM10 Antibody

Application Note :

ADAM10 antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 kDa in size corresponding to ADAM10 protein by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:20,000

Immunohistochemistry Dilution :

1:100-1:800

IF Microscopy Dilution :

1:250 - 1:1,000

Western Blot Dilution :

1:500 to 1:2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

ADAM10

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ADAM10 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

ADAM10 IgG fraction antibody is directed against human ADAM10 protein. The product was purified from monospecific antiserum by DEAE chromatography. A BLAST analysis was used to suggest cross-reactivity with ADAM10 protein from human, bovine and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against ADAM10 from mouse based on a single amino acid change relative to this sequence. Reactivity against homologues from other sources is not known.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

A disintegrin and metalloprotease domain 10 antibody, A Disintegrin And Metalloproteinase Domain 10 antibody, ADAM 10 antibody, ADAM metallopeptidase domain 10 antibody, CD 156c antibod, KUZ, MADM

Target Species :

Human

Background :

Proinflammatory cytokine tumor necrosis factor-α (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch, and its ligand delta, are all membrane-bound molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ) and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues. Anti-ADAM10 is ideal for investigators involved in NF-kappaB, Apoptosis, Cytokins and Growth factor research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O14672

NCBI :

4557251

Primary Image Name :

Anti-ADAM10 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage