S100 Protein Antibody | Rockland Immunochemicals 200-401-874 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

S100 Protein Antibody

catalog :

200-401-874

quantity :

500

price :

https://www.rockland.com/search/?searchString=200-401-874

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-401-874

Name :

Anti-S100 Protein (RABBIT) Antibody - 200-401-874

Display Name :

S100 Protein Antibody

Application Note :

This Protein A purified antibody has been tested for use in immunohistochemistry, and western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 11 kDa in size corresponding to S100 monomer by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

5.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

500

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:25,000

Immunohistochemistry Dilution :

1:200 - 1:2,000

Western Blot Dilution :

1:500 - 1:3,000

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgG

Gene Name :

S100A1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This Protein A purified antibody is directed against bovine S100 protein. The product was purified from monospecific antiserum by Protein A chromatography. A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

Human; Mouse; Rat; Bovine

Tested Applications :

IHC; WB

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-S100 Protein Antibody, S100 calcium-binding protein A1, S-100 protein subunit alpha, S-100 protein alpha chain

Target Species :

Bovine

Background :

S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - bb homodimer (S-100b) and ab heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke. Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2024 Price :

$525.00

UniProt :

P02639

NCBI :

NP_001092512.1

Primary Image Name :

Anti-S-100 Protein Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage