BCL2 Antibody | Rockland Immunochemicals 200-401-222 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

BCL2 Antibody

catalog :

200-401-222

quantity :

200 µg

price :

395.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Ramaswamy K, Forbes L, Minuesa G, Gindin T, Brown F, Kharas M, et al. Peptidomimetic blockade of MYB in acute myeloid leukemia. Nat Commun. 2018;9:110 pubmed publisher

product information

Catalog Number :

200-401-222

Name :

Anti-BCL2 (RABBIT) Antibody - 200-401-222

Display Name :

BCL2 Antibody

Application Note :

This purified IgG antibody against Human Bcl-2 has been tested for use in ELISA and immunoblotting. The antibody recognizes a 27 kDa band corresponding to human bcl-2. Anti-Bcl-2 shows perinuclear staining of Bcl-2. Reactivity in other immunoassays is unknown.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

2.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

200 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Western Blot Dilution :

1:500- 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

BCL2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This purified IgG antibody was prepared from rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 62-76 (RDPVARTSPLQTPAA) of human Bcl-2.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This purified IgG antibody was prepared from monospecific rabbit antiserum by Protein A chormatography. The antibody is directed against human Bcl-2 and is useful in determining its presence in various assays.

Species Reactivity :

Human, mouse, rat, bovine

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Bcl-2 antibody, Apoptosis regulator Bcl 2 antibody, Apoptosis regulator Bcl2 antibody, AW986256 antibody, B cell CLL/lymphoma 2 antibody, B cell leukemia/lymphoma 2 antibody, B cell lymphoma 2 antibody, Bcl 2 antibody

Target Species :

Human

Background :

Bcl-2 is a human proto-oncogene located chromosome 18. Its product is an integral membrane protein (also called Bcl-2) located in the membranes of the endoplasmic reticulum (ER), nuclear envelope, and in the outer membranes of the mitochondria. The gene was discovered as the translocated locus in a B-cell leukemia (hence the name). This translocation is also found in some B-cell lymphomas. In the cancerous B cells, the portion of chromosome 18 containing the BCL-2 locus has undergone a reciprocal translocation with the portion of chromosome 14 containing the antibody heavy chain locus. This t(14;18) translocation places the BCL-2 gene close to the heavy chain gene enhancer. This enhancer is very active in B cells and therefore results in high levels of Bcl-2 expression in these cells. High levels of the Bcl-2 protein protect the cells from early death by apoptosis. The Bcl-2 protein suppresses apoptosis by preventing the activation of the caspases that carry out the process. It is conceived that introduction of the Bcl-2 gene into the cells of injured tissue will reduce cell death and improve the clinical outcome of the injury. Anti-BCL-2 Antibody is useful for researchers interested in lymphoma, breast cancer, and apoptosis research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

395.00 USD

UniProt :

P10415

NCBI :

P10415.2

Primary Image Name :

Anti-Bcl-2 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage