product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Ferritin Antibody
catalog :
200-401-090-0100
quantity :
100
price :
499.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry
citations: 4
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:200; loading ...; fig 1f
Goodwin J, Dowdle W, Dejesus R, Wang Z, Bergman P, Kobylarz M, et al. Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9. Cell Rep. 2017;20:2341-2356 pubmed publisher
Mai T, Hamai A, Hienzsch A, Cañeque T, Müller S, Wicinski J, et al. Salinomycin kills cancer stem cells by sequestering iron in lysosomes. Nat Chem. 2017;9:1025-1033 pubmed publisher
Kimura T, Jia J, Kumar S, Choi S, Gu Y, Mudd M, et al. Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy. EMBO J. 2017;36:42-60 pubmed publisher
Mancias J, Pontano Vaites L, Nissim S, Biancur D, Kim A, Wang X, et al. Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis. elife. 2015;4: pubmed publisher
product information
Catalog Number :
200-401-090-0100
Name :
Anti-Ferritin (Human Spleen) (RABBIT) Antibody - 200-401-090-0100
Display Name :
Ferritin Antibody
Application Note :
Anti-Ferritin has been tested in ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/ml
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:5,000 - 1:20,000
Immunohistochemistry Dilution :
1:200 - 1:1,000
Western Blot Dilution :
1:5,000 - 1:2,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
FTH1
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Ferritin [Human Spleen]
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-Ferritin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].  Cross reactivity against Ferritin from other tissues and species may occur but have not been specifically determined.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Species Reactivity :
Human
Tested Applications :
ELISA; WB
Suggested Applications :
FC; IF; LFA; Multiplex
Storage :
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-Ferritin Antibody, FTHL6, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH
Target Species :
Human
Background :
Ferritin stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2025 Price :
499.00 USD
UniProt :
P02794
NCBI :
AAH66341.1
Primary Image Name :
WB - Ferritin Antibody
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.