NrCAM Antibody ATTO 700 | Rockland Immunochemicals 200-362-W74 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

NrCAM Antibody ATTO 700

catalog :

200-362-W74

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

Atto 700

clone name :

S364-51

reactivity :

mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-362-W74

Name :

Anti-NrCAM (MOUSE) Monoclonal Antibody ATTO 700 Conjugated - 200-362-W74

Display Name :

NrCAM Antibody ATTO 700

Application Note :

Anti-NrCAM ATTO 700 Conjugated Antibody is suitable for Western Blots and Immunocytochemistry. Expect a band approximately ~160kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

S364-51

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

AT700

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2a

Gene Name :

Nrcam

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-NrCAM Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 30-845 of mouse NrCAM.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

Anti-NrCAM Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. BLAST analysis suggests that it is 96% identical to rat, 91% identical to human and ~50% identical to Neurofascin.

Species Reactivity :

Mouse, Rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2a

Synonyms :

Neuronal cell adhesion molecule, Bravo, hBravo, KIAA0343, MGC138845, MGC138846, Neuronal surface protein Bravo, Ng CAM related, Ng-CAM-related, NgCAM related cell adhesion molecule, NgCAM-related cell adhesion molecule, Nr CAM, Nr-CAM

Target Species :

Mouse

Background :

Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. Anti-NrCam is ideal for research in Cancer and Neuroscience. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Extracellular

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

ATTO 700

UniProt :

Q810U4

NCBI :

NP_001139503

more info or order :

Rockland Immunochemicals product webpage