GFP Antibody Dylight™ 549 Conjugated | Rockland Immunochemicals 200-342-215 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

GFP Antibody Dylight™ 549 Conjugated

catalog :

200-342-215

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

DyLight549

clone name :

9F9.F9

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-342-215

Name :

Anti-GFP (MOUSE) Monoclonal Antibody DyLight™ 549 Conjugated - 200-342-215

Display Name :

GFP Antibody Dylight™ 549 Conjugated

Application Note :

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

9F9.F9

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

DL549

Size :

100 µg

Default Unit :

µg

FLISA Dilution :

>1:20,000

Fluorochrome Protein Value :

4.0

IF Microscopy Dilution :

>1:5,000

Western Blot Dilution :

1:10,000 - 1:25,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

GFP Dylight™ 549 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by Western blot. No reaction is seen against RFP.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

wt, rGFP, eGFP

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-GFP Antibody Dylight™ 549 Conjugation, Dylight™ 549 Conjugated mouse anti-GFP Antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background :

GFP monoclonal antibody is Dylight™ 549 Conjugated. Labeled GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.

Epitope Tag Type :

GFP

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

FLISA,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

DyLight™ 549

Primary Image Name :

Two-color Western Blot - TNFα and Tubulin

more info or order :

Rockland Immunochemicals product webpage