product summary
request information :
company name :
Rockland Immunochemicals
product type :
antibody
product name :
PhosphoThreonine Monoclonal Antibody IRDye800® Conjugated
catalog :
200-332-263
quantity :
100 µg
price :
Discontinued
clonality :
monoclonal
host :
mouse
conjugate :
IRDye 800
application :
western blot
more info or order :
Rockland Immunochemicals product webpage
image
image 1 :
Rockland Immunochemicals 200-332-263 image 1
Western Blot showing detection of alpha tubulin from HeLa and NIH/3T3. Protein was run on a 4-20% gel, transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, primary antibody was used at 1:2500 overnight at 4°C. IRDye800CW® secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.
product information
Catalog Number :
200-332-263
Name :
Anti-PhosphoThreonine (MOUSE) Monoclonal Antibody IRDye® 800 Conjugated - 200-332-263
Display Name :
PhosphoThreonine Monoclonal Antibody IRDye800® Conjugated
Application Note :
Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye® 800 and IRDye® 700DX antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. IRDye® 800 conjugates are optimized for the Odyssey® Infrared Imaging System developed by LI-COR. IRDye® 800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved when IRDye® 800 conjugates are used in conjunction with IRDye® 700DX or DyLight™680 conjugates. IRDye® 800 and IRDye® 700DX conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
18F6
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
IR800
Size :
100 µg
Default Unit :
µg
FLISA Dilution :
1:5,000 - 1:10,000
Fluorochrome Protein Value :
4.3
Western Blot Dilution :
1:10,000 - 1:25,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG1
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This protein A purified mouse monoclonal antibody reacts specifically with phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Subclass :
IgG1 kappa
Synonyms :
Immunofluorescence microscopy antibodies, phosphothreonine reactive antibody, monoclonal phosphoThreonine
Background :
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Low Endotoxin :
No
Sample Size :
No
Created On :
12/15/2011 7:07 PM
Modified On :
6/24/2015 1:14 PM
Application Text :
FLISA,Fluorochrome Protein,Western Blot
Category :
Secondary Antibodies
Conjugation Name :
IRDye® 800
2015 List Price :
315.00
2016 List Price :
Discontinued
Primary Image Name :
IR800CW - Western Blot
Primary Image Legend :
Western Blot showing detection of alpha tubulin from HeLa and NIH/3T3. Protein was run on a 4-20% gel, transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, primary antibody was used at 1:2500 overnight at 4°C. IRDye800CW® secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
P.O. Box 326
Gilbertsville, PA 19525
Info@rockland-inc.com
www.rockland-inc.com
1-610-369-1008
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.