product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD80 Allophycocyanin Antibody
catalog :
200-326-N74
quantity :
500 µL
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
2D10
reactivity :
human
application :
western blot, immunohistochemistry, flow cytometry
product information
Catalog Number :
200-326-N74
Name :
Anti-CD80 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N74
Display Name :
CD80 Allophycocyanin Antibody
Application Note :
Anti-CD80 is useful for Flow Cytometry, Immunohistochemistry, and Western Blot. Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
2D10
Concentration Definition :
Sufficient to run approximately 100 tests
Conjugation :
APC
Size :
500 µL
Default Unit :
ul
Flow Cytometry Dilution :
5 uL/test/1x10e5 to 1x10e8 cells
Fluorochrome Protein Value :
1-2
Immunohistochemistry Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG1
Gene Name :
CD80
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CD80 antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Allophycocyanin conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD80. Reactivity is observed against human CD80. Cross reactivity is observed with Rhesus. Reactivity with CD80 from other sources has not been tested. Anti-CD80 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
Species Reactivity :
Human
Stabilizer :
0.2% BSA (w/v)
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG1 kappa
Synonyms :
T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7, CD80, CD28LG, CD28LG1, LAB7
Target Species :
Human
Background :
CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Allophycocyanine
UniProt :
P33681
NCBI :
NP_005182.1
Primary Image Name :
Flow Cytometry of Mouse anti-CD80 Allophycocyanin
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.