CD27 Allophycocyanin Antibody | Rockland Immunochemicals 200-326-N65 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD27 Allophycocyanin Antibody

catalog :

200-326-N65

quantity :

500 µL

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

O323

reactivity :

human

application :

flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-326-N65

Name :

Anti-CD27 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N65

Display Name :

CD27 Allophycocyanin Antibody

Application Note :

Anti-CD27 is useful for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

O323

Concentration Value :

0.05 mg/mL

Concentration Definition :

Sufficient to run approximately 100 tests

Conjugation :

APC

Size :

500 µL

Default Unit :

Flow Cytometry Dilution :

5µL/test/1x10e5 to 1x10e8 cells

Fluorochrome Protein Value :

1-2

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG1

Gene Name :

CD27

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Allophycocyanin conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27. Reactivity is observed against human CD27, Baboon, Cynomolgus, and Rhesus. Cross reactivity with CD27 from other sources has not been tested. Anti-CD27 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Species Reactivity :

Human

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Subclass :

IgG1 kappa

Synonyms :

CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7

Target Species :

Human

Background :

CD27 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The CD27 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,

Category :

Primary Antibodies

Conjugation Name :

Allophycocyanine

UniProt :

P26842

NCBI :

NP_001233.1

Primary Image Name :

Flow Cytometry - Mouse anti-CD27 APC

more info or order :

Rockland Immunochemicals product webpage