product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD4 Allophycocyanin Antibody
catalog :
200-326-N18
quantity :
500 µL
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
OKT4
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-326-N18
Name :
Anti-CD4 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N18
Display Name :
CD4 Allophycocyanin Antibody
Application Note :
Anti-CD4 is useful for Immunoprecipitation, Immunofluorescence, Immunohistochemistry, and Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
OKT4
Concentration Value :
0.025 mg/mL
Concentration Definition :
Sufficient to run approximately 100 tests
Conjugation :
APC
Size :
500 µL
Default Unit :
ul
Flow Cytometry Dilution :
5µL/test/1x10e5 to 1x10e8 cells
Fluorochrome Protein Value :
1-2
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG2b
Gene Name :
CD4
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with CD4 antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Allophycocyanin conjugated CD4 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD4. Reactivity is observed against human CD4, Cynomolgus, Rhesus, and chimpanzee. This antibody recognizes a different epitope, and thus does not block binding of clone RPA-T4. Cross reactivity with CD4 from other sources has not been tested. Anti-CD4 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
Species Reactivity :
Human
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG2b kappa
Synonyms :
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, FITC anti-human CD4, RPA-T4, APC, T4
Target Species :
Human
Background :
The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The OKT4 antibody is widely used as a phenotypic marker for CD4 expression.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Allophycocyanine
UniProt :
P01730
NCBI :
NP_000607.1
Primary Image Name :
Flow Cytometry - Mouse anti-CD4 APC
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.