product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD3 Allophycocyanin Antibody
catalog :
200-326-N12
quantity :
500 µL
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
HIT3a
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry
product information
Catalog Number :
200-326-N12
Name :
Anti-CD3 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N12
Display Name :
CD3 Allophycocyanin Antibody
Application Note :
Anti-CD3 is useful for Immunoprecipitation, Immunohistochemistry, and Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
HIT3a
Concentration Definition :
Sufficient to run approximately 100 tests
Conjugation :
APC
Size :
500 µL
Default Unit :
ul
Flow Cytometry Dilution :
5 ul/1x10e6 cells or 100µL of whole blood
Fluorochrome Protein Value :
1-2
Immunohistochemistry Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG2a
Gene Name :
CD3E
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Allophycocyanin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3. Reactivity is observed against human CD3. Cross reactivity with CD3 from other sources has not been tested. Anti-CD3 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
Species Reactivity :
Human
Stabilizer :
0.2% BSA (w/v)
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG2a kappa
Synonyms :
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Target Species :
Human
Background :
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,Immunoprecipitation,
Category :
Primary Antibodies
Conjugation Name :
Allophycocyanine
UniProt :
P07766
NCBI :
NP_000724.1
Primary Image Name :
Flow Cytometry - Mouse anti-CD3 APC
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.