CD3 APC Antibody | Rockland Immunochemicals 200-326-M62 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD3 APC Antibody

catalog :

200-326-M62

quantity :

500 µL

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

OKT3

reactivity :

human

application :

flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-326-M62

Name :

Anti-CD3 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-M62

Display Name :

CD3 APC Antibody

Application Note :

Anti-CD3 is useful for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

OKT3

Concentration Value :

0.025 mg/mL

Concentration Definition :

Sufficient to run approximately 100 tests

Conjugation :

APC

Size :

500 µL

Default Unit :

Flow Cytometry Dilution :

10 µL/1x10^6 cells

Fluorochrome Protein Value :

1-2

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG2a

Gene Name :

CD3e

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Allophycocyanin conjugated CD3 Monoclonal Antibody was Protein G Purified and is directed against human CD3. Reactivity is observed against human CD3 and chimpanzee. Cross reactivity with CD3 from other sources has not been tested.

Species Reactivity :

human

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Subclass :

IgG2a kappa

Synonyms :

T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Target Species :

Human

Background :

CD3 (T3), a complex T cell marker, is known to associate noncovalently with the a/b or g/z heterodimer of the T cell antigen receptor (TCR) to form the most complex transmembrane (TM) receptor structures. Structurally, CD3 consists of four distinct membrane glycoprotein isoforms (CD3g, CD3e, CD3s and the zeta chain) to generate activation signals. CD3 is specially engaged in antigen recognition and is known to play an important role in mediating signals that are critical for T cell development in the thymus, proliferation, and induction of T cell-mediated immune responses against infectious agents and also in the differentiation of T cells into effector and memory populations. CD3 usually expresses in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, but cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. Apart from its role as an important marker in the classification of malignant lymphomas and lymphoid leukaemia, CD3 can also be useful for the identification of T cells in celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein. CD3 indirectly plays an important role in immunomodulation whereas the anti-CD3 antibody may be used in in vitro Treg assays to generate effector T cells.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,

Category :

Primary Antibodies

Conjugation Name :

Allophycocyanine

UniProt :

P07766

NCBI :

NP_000724.1

Primary Image Name :

Flow Cytometry - Mouse anti-HUMAN CD3 APC

more info or order :

Rockland Immunochemicals product webpage