product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD45RO Phycoerythrin Antibody
catalog :
200-308-N71
quantity :
500 µL
clonality :
monoclonal
host :
mouse
conjugate :
RPE
clone name :
UCHL1
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-308-N71
Name :
Anti-CD45RO (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N71
Display Name :
CD45RO Phycoerythrin Antibody
Application Note :
Anti-CD45RO is useful for Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, and Western Blot. Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
UCHL1
Concentration Value :
0.1 mg/mL
Concentration Definition :
Sufficient to run approximately 100 tests
Conjugation :
RPE
Size :
500 µL
Default Unit :
ul
Flow Cytometry Dilution :
5 uL/test/1x10e5 to 1x10e8 cells
Fluorochrome Protein Value :
1-2
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG2a
Gene Name :
PTPRC
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD45RO Antibody (Monoclonal) was produced by repeated immunizations with CD45RO antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Phycoerythrin conjugated CD45RO Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45RO. Reactivity is observed against human CD45RO. Cross reactivity is observed with Chimpanzee. Reactivity with CD45RO from other sources has not been tested. Anti-CD45RO is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Species Reactivity :
Human
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Subclass :
IgG2a kappa
Synonyms :
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, PTPRC , CD45
Target Species :
Human
Background :
The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and others.The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages and granulocytes. The antibody is reported to be cross-reactive for Chimpanzee CD45RO.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
R-Phycoerythrin (RPE)
UniProt :
P08575
NCBI :
NP_002829.3
Primary Image Name :
Flow Cytometry of Mouse anti-CD45RO PE - 200-308-N71
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.