CD45 Phycoerythrin Antibody | Rockland Immunochemicals 200-308-N68 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD45 Phycoerythrin Antibody

catalog :

200-308-N68

quantity :

500 µL

clonality :

monoclonal

host :

mouse

conjugate :

RPE

clone name :

HI30

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-308-N68

Name :

Anti-CD45 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N68

Display Name :

CD45 Phycoerythrin Antibody

Application Note :

Anti-CD45 is useful for Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

HI30

Concentration Definition :

Sufficient to run approximately 100 tests

Conjugation :

RPE

Size :

500 µL

Default Unit :

Flow Cytometry Dilution :

5 uL/test/1x10e5 to 1x10e8 cells

Fluorochrome Protein Value :

1-2

Immunohistochemistry Dilution :

User Optimized

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG1

Gene Name :

PTPRC

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD45 Antibody (Monoclonal) was produced by repeated immunizations with CD45 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Phycoerythrin conjugated CD45 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45. Reactivity is observed against human CD45. Cross reactivity is observed with Chimpanzee. Reactivity with CD45 from other sources has not been tested. Anti-CD45 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Species Reactivity :

Human

Stabilizer :

0.2% BSA (w/v)

Storage :

Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Subclass :

IgG1 kappa

Synonyms :

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, CD45, PTPRC

Target Species :

Human

Background :

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

R-Phycoerythrin (RPE)

UniProt :

P08575

NCBI :

NP_002829.3

Primary Image Name :

Flow Cytometry of Mouse anti-CD45 PE - 200-308-N68

more info or order :

Rockland Immunochemicals product webpage