CD28 Phycoerythrin Antibody | Rockland Immunochemicals 200-308-N66 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD28 Phycoerythrin Antibody

catalog :

200-308-N66

quantity :

500 µL

clonality :

monoclonal

host :

mouse

conjugate :

RPE

clone name :

CD28.2

reactivity :

human

application :

immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-308-N66

Name :

Anti-CD28 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N66

Display Name :

CD28 Phycoerythrin Antibody

Application Note :

Anti-CD28 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

CD28.2

Concentration Value :

0.05 mg/mL

Concentration Definition :

Sufficient to run approximately 100 tests

Conjugation :

RPE

Size :

500 µL

Default Unit :

Flow Cytometry Dilution :

5µL/test/1x10e5 to 1x10e8 cells

Fluorochrome Protein Value :

1-2

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG1

Gene Name :

CD28

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD28 Antibody (Monoclonal) was produced by repeated immunizations with CD28 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Phycoerythrin conjugated CD28 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD28. Reactivity is observed against human CD28, Baboon, Chimpanzee, Cynomolgus, and Rhesus. Cross reactivity with CD28 from other sources has not been tested. Anti-CD28 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Species Reactivity :

Human

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Subclass :

IgG1 kappa

Synonyms :

T-cell-specific surface glycoprotein CD28, TP44, CD28

Target Species :

Human

Background :

The CD28 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA. The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,Immunoprecipitation,

Category :

Primary Antibodies

Conjugation Name :

R-Phycoerythrin (RPE)

UniProt :

P10747

NCBI :

NP_001230006.1

Primary Image Name :

Flow Cytometry - Mouse anti-CD28 PE

more info or order :

Rockland Immunochemicals product webpage