CD11b Phycoerythrin Antibody | Rockland Immunochemicals 200-308-N20 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD11b Phycoerythrin Antibody

catalog :

200-308-N20

quantity :

500 µL

clonality :

monoclonal

host :

mouse

conjugate :

RPE

clone name :

ICRF44

reactivity :

human

application :

immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-308-N20

Name :

Anti-CD11b (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N20

Display Name :

CD11b Phycoerythrin Antibody

Application Note :

Anti-CD11b is useful for Flow Cytometry, Immunofluorescence, and Immunoprecipitation. Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

ICRF44

Concentration Value :

0.2 mg/mL

Concentration Definition :

Sufficient to run approximately 100 tests

Conjugation :

RPE

Size :

500 µL

Default Unit :

Flow Cytometry Dilution :

5µL/test/1x10e5 to 1x10e8 cells

Fluorochrome Protein Value :

1-2

IF Microscopy Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG1

Gene Name :

ITGAM

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD11b. Reactivity is observed against human CD11b, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Swine. Cross reactivity with CD11b from other sources has not been tested. Anti-CD11b is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Species Reactivity :

Human

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Subclass :

IgG1 kappa

Synonyms :

Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, CR3A

Target Species :

Human

Background :

The ICRF44 antibody reacts with human CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The ICRF44 antibody is widely used as a marker for CD11b expression on macrophages, granulocytes, and subsets of NK cells.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,IF Microscopy,Immunoprecipitation,

Category :

Primary Antibodies

Conjugation Name :

R-Phycoerythrin (RPE)

UniProt :

P11215

NCBI :

NP_000623.2

Primary Image Name :

Flow Cytometry - Mouse anti-CD11b PE

more info or order :

Rockland Immunochemicals product webpage