AKT3 PE Antibody | Rockland Immunochemicals 200-308-J36 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

AKT3 PE Antibody

catalog :

200-308-J36

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

RPE

clone name :

9A5.H9.G7

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-308-J36

Name :

Anti-AKT3 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-J36

Display Name :

AKT3 PE Antibody

Application Note :

Anti-AKT3 PE Antibody is suitable for Flow Cytometry, ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Buffer :

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

9A5.H9.G7

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

RPE

Size :

50 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Flow Cytometry Dilution :

User Optimized

Fluorochrome Protein Value :

1-2

Immunohistochemistry Dilution :

User optimized

IF Microscopy Dilution :

User optimized

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

AKT3

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.

Packing Type :

Wet Ice

Physical State :

Lyophilized

Purity and Specificity :

Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

50µL

Species Reactivity :

Human, Mouse, Rat

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Subclass :

IgG1 kappa

Synonyms :

Mouse anti-AKT3 antibody PE conjugation, phycoerythtin conjugated Mouse anti-AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

Target Species :

Human

Background :

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT3 (MOUSE) PE conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

R-Phycoerythrin (RPE)

UniProt :

Q9Y243

NCBI :

NP_001193658.1

Primary Image Name :

AKT3 Phycoerythrin Conjugated Monoclonal Antibody - Flow Cytometry

more info or order :

Rockland Immunochemicals product webpage