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company name :
Rockland Immunochemicals
product type :
antibody
product name :
DMPO Antibody
catalog :
200-306-F21
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
N1664A
application :
immunohistochemistry, immunoprecipitation
more info or order :
product information
Catalog Number :
200-306-F21
Name :
Anti-DMPO (MOUSE) Monoclonal Antibody Biotin Conjugated
Display Name :
DMPO Antibody
Application Note :
Anti-DMPO Antibody is suitable for use in IP, IF microscopy, IHC and WB. Expect a band approximately ~90kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4
Clonality :
Monoclonal
Clone ID :
N1664A
Concentration Value :
1mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
DMPO Antibody was produced in mice by repeated immunizations with 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.05% (w/v) Sodium Azide
Purity and Specificity :
Anti-DMPO Antibody was purified by Protein G chromatography. DMPO is species independant. This antibody recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA. Cross-reactivity with DMPO from other sources has not been determined. Neuroscience research.
Species Reactivity :
pan
Stabilizer :
50% (v/v) Glycerol
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG
Synonyms :
5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide
Target Species :
Adenovirus
Background :
The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
Immunohistochemistry,IF Microscopy,Immunoprecipitation,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
Primary Image Name :
DMPO Immunofluorescence
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.