ATF4 Antibody Peroxidase | Rockland Immunochemicals 200-303-W61 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ATF4 Antibody Peroxidase

catalog :

200-303-W61

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

S360A-24

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-303-W61

Name :

Anti-ATF4 (MOUSE) Monoclonal Antibody Peroxidase Conjugated - 200-303-W61

Display Name :

ATF4 Antibody Peroxidase

Application Note :

Anti-ATF4 HRP Conjugated Antibody is suitable for Western Blots and Immunocytochemistry. Expect a band approximately ~60 kDa on specific lysates or tissues. Does not cross react with ATF5. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

S360A-24

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000

Immunohistochemistry Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2a

Gene Name :

ATF4

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-ATF4 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 25-327 of human ATF4.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

Anti-ATF4 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. BLAST analysis suggests that it is 85% identical to mouse, 86% identical to rat and <50% identical to ATF5. Will not cross react with ATF5.

Species Reactivity :

Human, Rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2a

Synonyms :

Activating Transcription Factor 4, ATF 4, ATF4 protein, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB 2, CREB-2, CREB2, Cyclic AMP dependent transcription factor ATF 4

Target Species :

Human

Background :

Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation. Anti-ATF4 is ideal for researchers interested in Cell Signaling, Oncology, Cell Differentiation, and Apoptosis; relevant pathways include MAPK signaling pathways, Activation of cAMP-Dependent PKA, CREB pathways, GPCR pathways and Rho Family GTPases.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Peroxidase (Horseradish)

UniProt :

P18848

more info or order :

Rockland Immunochemicals product webpage