NK1.1 Fluorescein Antibody | Rockland Immunochemicals 200-302-N93 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

NK1.1 Fluorescein Antibody

catalog :

200-302-N93

quantity :

500 µg

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

PK136

reactivity :

mouse

application :

immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-302-N93

Name :

Anti-NK1.1 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N93

Display Name :

NK1.1 Fluorescein Antibody

Application Note :

Anti-NK1.1 is useful for Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

PK136

Concentration Value :

0.5 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

FITC

Size :

500 µg

Default Unit :

µg

Flow Cytometry Dilution :

10 µL/10^6 cells (0.1 µg)

Fluorochrome Protein Value :

Sufficient to run approximately 500 tests

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG2a

Gene Name :

Klrb1c

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-NK1.1 Antibody (Monoclonal) was produced by repeated immunizations with NK1.1 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Fluorescein conjugated NK1.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse NK1.1. Cross reactivity with NK1.1 from other sources has not been tested. Anti-NK1.1 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Species Reactivity :

Mouse

Stabilizer :

0.1% Gelatin

Storage :

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Subclass :

IgG2a kappa

Synonyms :

Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c

Target Species :

Mouse

Background :

The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,

Other :

Sufficient to run approximately 500 tests

Category :

Primary Antibodies

Conjugation Name :

Fluorescein (FITC)

UniProt :

P27814

NCBI :

NP_001153376.1

Primary Image Name :

Flow Cytometry of anti-NK1.1 FITC - 200-302-N93

more info or order :

Rockland Immunochemicals product webpage