product summary
Loading...
company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD69 Fluorescein Antibody
catalog :
200-302-N73
quantity :
500 µL
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
FN50
reactivity :
human
application :
immunohistochemistry, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-302-N73
Name :
Anti-CD69 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N73
Display Name :
CD69 Fluorescein Antibody
Application Note :
Anti-CD69 is useful for Flow Cytometry, Immunohistochemistry, and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
FN50
Concentration Definition :
Sufficient to run approximately 100 tests
Conjugation :
FITC
Size :
500 µL
Default Unit :
ul
Flow Cytometry Dilution :
5 uL/test/1x10e5 to 1x10e8 cells
Fluorochrome Protein Value :
4-6
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG1
Gene Name :
CD69
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD69 Antibody (Monoclonal) was produced by repeated immunizations with CD69 antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Fluorescein conjugated CD69 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD69. Reactivity is observed against human CD69. Cross reactivity is observed with Chimpanzee, Baboon, Cynomolgus, Rhesus, and Pigtailed Macaque. Reactivity with CD69 from other sources has not been tested. Anti-CD69 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Species Reactivity :
Human
Stabilizer :
0.2% BSA (w/v)
Storage :
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Subclass :
IgG1 kappa
Synonyms :
Early activation antigen CD69, Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69, CLEC2C
Target Species :
Human
Background :
CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,IF Microscopy,
Category :
Primary Antibodies
Conjugation Name :
Fluorescein (FITC)
UniProt :
Q07108
NCBI :
NP_001772.1
Primary Image Name :
Flow Cytometry of Mouse anti-CD69 FITC - 200-302-N73
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.