product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD27 Fluorescein Antibody
catalog :
200-302-N65
quantity :
500 µL
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
O323
reactivity :
human
application :
flow cytometry
product information
Catalog Number :
200-302-N65
Name :
Anti-CD27 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N65
Display Name :
CD27 Fluorescein Antibody
Application Note :
Anti-CD27 is useful for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
O323
Concentration Definition :
Sufficient to run approximately 100 tests
Conjugation :
FITC
Size :
500 µL
Default Unit :
ul
Flow Cytometry Dilution :
5 ul/1x10e6 cells or 100µL of whole blood
Fluorochrome Protein Value :
4-6
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG1
Gene Name :
CD27
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Fluorescein conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27. Reactivity is observed against human CD27, Baboon, Cynomolgus, Rhesus, and Squirrel Monkey. Cross reactivity with CD27 from other sources has not been tested. Anti-CD27 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Species Reactivity :
Human
Stabilizer :
0.2% BSA (w/v)
Storage :
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Subclass :
IgG1 kappa
Synonyms :
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
Target Species :
Human
Background :
CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-kB and SAPK/JNK activation.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,
Category :
Primary Antibodies
Conjugation Name :
Fluorescein (FITC)
UniProt :
P26842
NCBI :
NP_001233.1
Primary Image Name :
Flow Cytometry - Mouse anti-CD27 FITC
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.