CD8a Fluorescein Antibody | Rockland Immunochemicals 200-302-N61 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD8a Fluorescein Antibody

catalog :

200-302-N61

quantity :

500 µL

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

Hit8a

reactivity :

human

application :

immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-302-N61

Name :

Anti-CD8a (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N61

Display Name :

CD8a Fluorescein Antibody

Application Note :

Anti-CD8a is useful for Immunohistochemistry and Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

HIT8a

Concentration Definition :

Sufficient to run approximately 100 tests

Conjugation :

FITC

Size :

500 µL

Default Unit :

Flow Cytometry Dilution :

5 ul/1x10e6 cells or 100µL of whole blood

Fluorochrome Protein Value :

4-6

Immunohistochemistry Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG1

Gene Name :

CD8A

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Fluorescein conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a. Reactivity is observed against human CD8a, Marmoset, and chimpanzee. This clone recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a. Cross reactivity with CD8a from other sources has not been tested. Anti-CD8a is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Species Reactivity :

Human

Stabilizer :

0.2% BSA (w/v)

Storage :

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Subclass :

IgG1 kappa

Synonyms :

T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a

Target Species :

Human

Background :

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,

Category :

Primary Antibodies

Conjugation Name :

Fluorescein (FITC)

UniProt :

P01732

NCBI :

NP_001139345.1

Primary Image Name :

Flow Cytometry - Mouse anti-CD8a FITC

more info or order :

Rockland Immunochemicals product webpage