CD47 Fluorescein Antibody | Rockland Immunochemicals 200-302-M68 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD47 Fluorescein Antibody

catalog :

200-302-M68

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

B6H12.2

reactivity :

human

application :

immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-302-M68

Name :

Anti-CD47 (MOUSE) Monoclonal Antibody Fluorescein Conjugated - 200-302-M68

Display Name :

CD47 Fluorescein Antibody

Application Note :

Anti-CD47 is useful for Flow Cytometry (Cell Surface) and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Buffer :

0.01 M Tris Cl, 0.15 M Sodium Chloride, pH 8.0

Clonality :

Monoclonal

Clone ID :

B6H12.2

Concentration Value :

0.75 mg/ml

Concentration Definition :

Sufficient to run approximately 25 tests

Conjugation :

FITC

Size :

100 µL

Default Unit :

Flow Cytometry Dilution :

10 µL/5 x 10^5 cells (0.5 µg)

Fluorochrome Protein Value :

2-8

Immunohistochemistry Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG1

Gene Name :

CD47

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD47 Antibody (Monoclonal) was produced by repeated immunizations with CD47 antigen.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Fluorescein conjugated CD47 Monoclonal Antibody was Protein G Purified and is directed against human CD47. Reactivity is observed against human CD47. Cross reactivity with CD47 from other sources has not been tested.

Species Reactivity :

Human

Storage :

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Subclass :

IgG1

Synonyms :

Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47

Target Species :

Human

Background :

CD47, originally named integrin-associated protein (IAP), is a 50-kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-a, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,

Category :

Primary Antibodies

Conjugation Name :

Fluorescein (FITC)

UniProt :

Q08722

NCBI :

NP_001768.1

Primary Image Name :

Flow Cytometry - Mouse anti-HUMAN CD47 FITC

more info or order :

Rockland Immunochemicals product webpage