APAF1 Antibody [5E11] | Rockland Immunochemicals 200-301-Y28 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

APAF1 Antibody [5E11]

catalog :

200-301-Y28

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[5E11]

reactivity :

human, mouse, rat

application :

ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-Y28

Name :

Anti-APAF1 (MOUSE) Monoclonal Antibody - 200-301-Y28

Display Name :

APAF1 Antibody [5E11]

Application Note :

Anti-Apaf-1 Antibody has been tested for use in ELISA, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 142 kDa in Western Blots of specific cell lysates and tissues. Validated in human samples. All other applications and species not yet tested.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

[5E11]

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Immunohistochemistry Dilution :

0.5 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Gene Name :

APAF1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Apaf1 antibody was produced by repeated immunizations in mice with peptide corresponding to amino acids near the C-terminus of human Apaf1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-Apaf-1 Monoclonal Antibody was Protein A purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with Apaf-1 [5E11] from other sources has not been determined.

Species Reactivity :

Human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Apaf-1 Antibody [5E11] , CED4, APAF-1

Target Species :

Human

Background :

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O14727

NCBI :

AAC51678

Primary Image Name :

Immunohistochemistry of Apaf-1 Antibody

more info or order :

Rockland Immunochemicals product webpage