5-hmc Antibody | Rockland Immunochemicals 200-301-V06 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

5-hmc Antibody

catalog :

200-301-V06

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

application :

ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-V06

Name :

Anti-5-hydroxymethylcytosine (MOUSE) Monoclonal Antibody - 200-301-V06

Display Name :

5-hmc Antibody

Application Note :

Anti-5hmC Antibody is suitable for Dot Blotting, ELISA, Hydroxymethylated DNA Immunoprecipitation Sequencing and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ELISA Dilution :

1:500

IF Microscopy Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-5hmC Antibody was produced in mice by repeated immunization with 5-hydroxymethlcytosine.

Other Performance Data :

hMeDIP: 2.5μg per IP. Dot Blot: 2 μg/ml

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-5hmC Antibody was purified by Protein A chromatography. Cross-reactivity with 5hmC from other sources has not been determined.

Species Reactivity :

Human, Mouse, Other (wide range)

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

5-hydroxymethylcytosine

Target Species :

Human

Background :

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells). Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies. Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5hmC Antibody is ideal for research in Epigenetics and Gene Expression.

Immunogen Type :

Other

Low Endotoxin :

Sample Size :

Application Text :

ELISA,IF Microscopy,

Other :

hMeDIP: 2.5μg per IP. Dot Blot: 2 μg/ml

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

Primary Image Name :

Mouse Anti-5-hydroxymethylcytosine Antibody immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage