AGO Antibody | Rockland Immunochemicals 200-301-V04 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

AGO Antibody

catalog :

200-301-V04

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A8

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-V04

Name :

Anti-AGO (Argonautes) (MOUSE) Monoclonal Antibody - 200-301-V04

Display Name :

AGO Antibody

Application Note :

Anti-Ago Antibody is suitable for HITS-CLIP (High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation), Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 97.2 kDa in the appropriate cell lysate or extract.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

2A8

Concentration Value :

2.39 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

Immunohistochemistry Dilution :

1:500

IF Microscopy Dilution :

1:500

Immunoprecipitation Dilution :

8 μg

Western Blot Dilution :

1:300 - 1:500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

AGO2

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Ago Antibody was produced in mice by repeated immunization with human Ago2 protein (fusion protein containing amino acids 47-859 of human Ago2).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-Ago Antibody was purified by Protein A chromatography. This 2A8 monoclonal antibody recognizes all 4 human and mouse Ago proteins while having higher affinity for Ago2. Cross-reactivity with Ago2 from other sources has not been determined.

Species Reactivity :

Human, Mouse, Other (wide range)

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, eIF-2C2, eIF2C 2, PAZ Piwi domain protein, PPD, Protein slicer

Target Species :

Human

Background :

Members of the Argonaute (Ago) protein family are internal to RISC function. Argonaute proteins bind to mature microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Argonautes are needed for miRNA-induced silencing and contain two conserved RNA binding domains: a PAZ domain that can bind the single stranded 3’ end of the mature miRNA and a PIWI domain that structurally resembles ribonuclease-H and functions to interact with the 5’ end of the guide strand. Some argonautes, for example human Ago2, cleave target transcripts directly; argonautes may also recruit additional proteins to achieve translational repression. The human genome encodes eight Argonaute proteins divided by sequence similarities into two families: AGO (with four members present in all mammalian cells and called E1F2C/hAgo in humans), and PIWI (found in the germ line and hematopoietic stem cells). Anti-Ago Antibody is ideal for research in Alternative Splicing, Gene Expression and Gene Silencing.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Other :

HITS-CLIP: User Optimized Dilution

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9UKV8

NCBI :

NP_001158095

Primary Image Name :

Co-immunoprecipitation of Anti-Ago antibody

more info or order :

Rockland Immunochemicals product webpage