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company name :
Rockland Immunochemicals
product type :
antibody
product name :
5-mC Antibody
catalog :
200-301-U98
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
application :
immunoprecipitation
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-301-U98
Name :
Anti-5-mC (MOUSE) Monoclonal Antibody - 200-301-U98
Display Name :
5-mC Antibody
Application Note :
Anti-5-mC Antibody is suitable for Dot Blotting, Fluorescence In Situ Hybridization (FISH), Immunofluorescence and Methylated DNA Immunoprecipitation (MeDIP). Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Concentration Value :
2.4 mg/ml
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
50 µg
Default Unit :
µg
IF Microscopy Dilution :
1:500
Immunoprecipitation Dilution :
1-2 μg/IP
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG1
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methyl Cytidine.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.05% (w/v) Sodium Azide
Purity and Specificity :
Anti-5-mC Antibody was purified by Protein A chromatography. Cross-reactivity with 5-mC from other sources has not been determined.
Species Reactivity :
Human, Mouse, Other (wide range)
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG1
Synonyms :
5-methylcytidine, 5-methyl Cytidine
Target Species :
Human
Background :
5-methyl Cytidine is a modified nucleotide found in the DNA of both plants and vertebrates. Anti-5-mC Antibody is ideal for research in Epigenetics, specifically DNA methylation, due to its applicability to methylation analysis.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
IF Microscopy,Immunoprecipitation,
Other :
FISH dilution: 1:200 - 1:1,000. Dot Blot: 1:600
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
Primary Image Name :
Immunofluorescence Microscopy of anti-5-mC antibody
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.